Journal: PeerJ

Article Title: Identification of potential insect ecological interactions using a metabarcoding approach

doi: 10.7717/peerj.18906

Figure Lengend Snippet: Target regions for analysis of insect samples including Lymantria dispar dispar (spongy moth), Agrilus planipennis (emerald ash borer), and larva from native pollinators. All primers were subsequently modified with CS1/CS2 tails for sequencing using the Illumina MiSeq platform with the exception of SSU_F_07/SSU_R_26 which were modified following Hebert et al. (2025) for nanopore sequencing.

Article Snippet: Because the 18S amplicons exceed the length which can be processed on Illumina platforms (>900 bp for the target nematodes), 18S emerald ash borer amplicons were sequenced using the MinION platform (Oxford Nanopore, Oxford, UK) with library prep carried out as described in and run on a Flongle flow cell.

Techniques: Modification, Sequencing, Nanopore Sequencing, Amplification

Journal: PeerJ

Article Title: Identification of potential insect ecological interactions using a metabarcoding approach

doi: 10.7717/peerj.18906

Figure Lengend Snippet: (A) In addition to the amplification of host DNA, COI primers co-amplified four orders of insects as minor background signals. (B) ITS primers targeting primarily fungal DNA recovered 11 orders in the DNA samples of larvae, pupae, and adults. (C) 16S primers targeting the microbiome found an increasingly complex microbial community with life stage. (D) 18S primers amplified the widest range of taxa including a series of nematodes which are likely parasites. Low taxonomic recovery from larva size 3 specimens likely reflects the small sample size.

Article Snippet: Because the 18S amplicons exceed the length which can be processed on Illumina platforms (>900 bp for the target nematodes), 18S emerald ash borer amplicons were sequenced using the MinION platform (Oxford Nanopore, Oxford, UK) with library prep carried out as described in and run on a Flongle flow cell.

Techniques: Amplification